lact c2 ezrin afbd yfp (Proteintech)
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Proteintech
lact c2 ezrin afbd yfp
Lact C2 Ezrin Afbd Yfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lact+c2+ezrin+afbd+yfp/Ezrin+Antibody/pmc06879320-82-0-5
Average 93 stars, based on 30 article reviews
Lact C2 Ezrin Afbd Yfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lact+c2+ezrin+afbd+yfp/Ezrin+Antibody/pmc06879320-82-0-5
Average 93 stars, based on 30 article reviews
lact c2 ezrin afbd yfp - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading"
Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading
Journal: Cell
doi: 10.1016/j.cell.2019.04.037
Figure Legend Snippet: (A and B) Schematic of the model transmembrane protein FRTM-Ezrin-AFBD (A) and the mutant FRTM-Ezrin-R579A (FRTM-Ez-AFBD*) (B) that impairs Ezrin-AFBD ability to interact with actin . (C–F) Representative intensity and steady-state anisotropy images and scatter dot plots with mean anisotropy of ROIs obtained from CHO cells stably expressing either FRTM-Ez-AFBD or FRTM-Ez-AFBD* as indicated. The cells were labeled with fluorescent folate, Pteroyl-lysyl-Bodipy(PLB) and plated on FN (blue, green) or glass (red, orange) prior to imaging in the absence (C and D) or after pre-treatment (E and F) with either 20 μM PP2 and 10 μM PF-573228 (red) or 10 μM SMIFH2 (green) or with the vehicle (DMSO; blue). (G–I) Schematic (G) of the supported lipid bilayer functionalized with cRGD that was prepared either on plain (continuous SLB; top) or on 5-nm-tall and 100-nm-wide chromium patterned (nanopatterned SLB, bottom) glass surfaces. (H and I) GFP-GPI-expressing CHO cells plated on glass (red) or on FN (blue) or treated with 10 mM mβCD on FN (green) or plated on either continuous SLBs with mobile ligand (magenta) or SLBs assembled on chromium nano-patterned surfaces. ROIs were drawn either on the pattern (orange) where the ligand is transiently immobile or from regions outside (brown) where the ligand is mobile. Scale bar 10μm in all panels. All error bars represent SD. n.s. p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Sample size and p values are provided in . See also .
Techniques Used: Mutagenesis, Stable Transfection, Expressing, Labeling, Imaging
Figure Legend Snippet: (A–J) Representative intensity and steady-state anisotropy images (A, C, E, G, and I) and scatter dot plot with mean anisotropy values (B, D, F, H, and J) of ROIs obtained from (A and B) vinculin-deficient cells (Vin −/− ) transfected with GFP-GPI (blue, orange) or co-transfected with mCherry-vinculin (+Vin-WT; red, green) and plated on FN or subsequently treated with 10 mM mβCD (orange, green). (C and D) Talin1-deficient cells without (Talin1 −/− ; blue, red) or with co-transfection with Talin2 shRNA (+Talin2 shR; green, orange) and re-plated onto FN after labeling with Alexa-568-FLAER prior to (blue, green) or post-treatment with 10 mM mβCD (red, orange). (E and F) Vin −/− cells alone (blue) or transiently transfected with GFP tagged Vin-WT (green), Vin-A50I (orange), or Vin-A50I-CA (brown) and plated onto FN after labeling with Alexa-568-FLAER. (G and H) Vin −/− cells were transiently transfected with FRTM-Ez-AFBD (FR-EZ; green) or with FR-Ez-AFBD* mutant (FR-EZ*; red), without (open circles) or with Vin-WT (closed circles) and re-plated onto FN after labeling with PLB. (I and J) Vin −/− cells alone (blue) or transfected with Lact C2-Ez-AFBD YFP (red) were labeled with Alexa-568-FLAER and re-plated on FN and directly labeled or treated with 10 mM mβCD (+mβCD; green). Dotted magenta lines in all images outline the transfected cells expressing the indicated constructs. Scale bar, 10 μm in all panels. All error bars represent SD. n.s. p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Sample size and p values are provided in . See also .
Techniques Used: Transfection, Cotransfection, shRNA, Labeling, Mutagenesis, Expressing, Construct
Figure Legend Snippet: Key Resources Table
Techniques Used: Purification, Transduction, Recombinant, Clinical Proteomics, Avidin-Biotin Assay, Membrane, Transfection, Plasmid Preparation, Stable Transfection, Expressing, Mutagenesis, Derivative Assay, Control, Construct, shRNA, Software
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Mutagenesis:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Stable Transfection:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Expressing:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Labeling:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Imaging:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Transfection:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Cotransfection:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , shRNA:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Construct:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Purification:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Transduction:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Recombinant:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Clinical Proteomics:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Avidin-Biotin Assay:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Membrane:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Plasmid Preparation:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Derivative Assay:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Control:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , Software:Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading Article Snippet: Lact C2 Ezrin AFBD-YFP , |